Date of Award
Bachelor of Science
Susan M. Bush
Lisa-Anne A. Foster
Robert J. Fleming and Claire T. Fournier
The gene CDT1 was first discovered in the crabgrass Digitaria cilaris to confer cadmium tolerance. Five homologs of this gene exist in rice (Oryza sativa), one of which, OsCDT3, was found to confer aluminum tolerance, instead of cadmium tolerance. The homolog of this gene in Arabidopsis thaliana (AtCDT1), is currently under study. Arabidopsis plants carrying a T-DNA insertion mutation in this gene were found to overexpress AtCDT1 in response to cadmium, but not to aluminum, suggesting that AtCDT1 is cadmium responsive, not aluminum responsive. This research study aims to learn more about the AtCDT1 gene and its potential involvement in tolerance to aluminum toxicity. The current study proposes a reporter construct to show where in the plant and when during development the AtCDT1 gene is expressed in Arabidopsis, with predictions that it is expressed in the cell membrane of cells in the roots, and throughout development of the Arabidopsis plant. Preliminary CRISPR constructs were designed to be used in further research to design a knockout line of Arabidopsis for the AtCDT1 gene, to determine its potential function in tolerance to only aluminum, only cadmium, both, or neither of these metals.
McCurry, Hannah, "Designing a Reporter Construct and CRISPR Construct to Identify the Role of AtCDT1 in Aluminum and Cadmium Tolerance in Arabidopsis thaliana". Senior Theses, Trinity College, Hartford, CT 2020.
Trinity College Digital Repository, https://digitalrepository.trincoll.edu/theses/846